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As jamerack says, the thickness and material of different strings does make the same note sound different. Playing harmonics will even this out quite well. You'll still get accurate notes, but they'll tend to sound more similar. Have a go at tuning using harmonics - it's been covered in other questions/answers here. Some disagree that it's accurate, all I ...


The gauge and material of the strings gives the same note a different timbre on different strings. If you are hearing different pitches, then it may be a product of an untrained ear. The longer you play for, the better your ear becomes and you will be able to better hear pitches and tonality.

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