That depends totally on the listener.
Many musicians can tell chords apart by their absolute frequency. Some of them strongly associate different emotions or impressions with specific chords, others don't.
Many other people cannot different minor chords apart, but they can tell major from minor. Again, some of them react strongly to minor vs. major chords, others don't have any particular reaction beyond "Oh yeah, this is minor, isn't it."
And of course, may people cannot tell a consonance from a dissonance at all and may react to loudness, instrumentation, beat etc. instead.
To make things more complicated, it is altogether possible to have different reactions to different stimuli without being able to consciously distinguish them (e.g. in an exam). That applies to music just as well as other sensations.
That said, the question whether different key have inherent characteristics, and which ones, has a long, disputed and fascinating history. Here is some info from wikipedia: