For background, I am not a musician, but I know a few things from physics. Physics tells us that we should perhaps expect that if your room changes, then you might need to tune your instruments differently - is this something that you can experience in real life? For instance, in a larger room, are notes more 'flat' than usual? (or the other way around perhaps?)
As another consideration, the characteristic wavelengths of some notes will be on the order of human scales (for instance, in usual conditions you can expect middle C to have a wavelength of around a meter and a half). In this situation, it seems that you might even have a phenomena where if people in the audience are all seated approximately this far apart from each other, you would hear a boost at this wavelength(?) Is this something that is ever observed? Do noise-cancelling materials pasted onto the walls largely mitigate both of these effects?